解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::A new fluorescence method using the dye FM1-43 was used to examine exocytotic release from hippocampal synaptosomes. Nitric oxide caused a marked transient stimulation of vesicle release. Several structurally unrelated nitric oxide donors, sodium nitroprusside, S-nitroso-N-acetylpenicillamine, 3-morpholino-sydnonimine...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(94)90440-5
更新日期:1994-06-01 00:00:00
abstract::In cultured rat embryonic septal nuclei with adjacent basal forebrain, murine interferon-gamma (IFN gamma) produces a striking increase in choline acetyltransferase (ChAT) activity and mRNA. The effect of IFN gamma on cholinergic differentiation is more potent in E14 cultures than in older cultures. IFN gamma does not...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(94)90322-0
更新日期:1994-05-01 00:00:00
abstract::Acetylcholinesterase (AChE) density at the neuromuscular junction of frog cutaneous pectoris muscle was determined by electron microscope autoradiography and biochemistry to be approximately 600 sites micron-2 of postsynaptic area, approximately 4-fold lower than all previous reports (mouse), whereas the hydrolytic tu...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(94)90331-x
更新日期:1994-04-01 00:00:00
abstract::Dynamic rearrangements of axon terminal arbors may be critical for establishing appropriate connections in the developing nervous system. Here, the changes in complex retinal axon arbors in the tecta of live Xenopus larvae were followed during the formation of the topographic retinotectal projection. Three-dimensional...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(94)90343-3
更新日期:1994-04-01 00:00:00
abstract::The structural basis for the synthesis of specific proteins within distinct intraneuronal compartments is unknown. We studied the distribution of poly(A) mRNA within cultured cerebrocortical neurons using high resolution in situ hybridization to identify cytoskeletal components that may anchor mRNA. After 1 day in cul...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(94)90213-5
更新日期:1994-03-01 00:00:00
abstract::Proteolipid protein (PLP) is an integral membrane protein of CNS myelin. Mutations of the X chromosome-linked PLP gene cause glial cell death and myelin deficiency in jimpy mice and other neurological mutants. As part of an attempt to rescue these mutants by transgenic complementation, we generated normal mouse lines ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(94)90214-3
更新日期:1994-03-01 00:00:00
abstract::mRNAs for AMPA- and kainate-preferring glutamate receptor subunits are expressed abundantly in the CNS, yet functional studies of neurons and glia from brain suggest selective expression of AMPA receptors. We now show that glial cells of the O-2A lineage express rapidly desensitizing responses to kainate, mRNAs for Gl...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(94)90277-1
更新日期:1994-02-01 00:00:00
abstract::In response to changes in membrane potential, voltage-dependent ion channel proteins undergo conformational rearrangements that lead to channel opening. These rearrangements move a net charge, measured as "gating current", across the membrane. Here we characterize the effects of the pharmacological blocker 4-aminopyri...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(94)90273-9
更新日期:1994-02-01 00:00:00
abstract::Serotonergic neurons are thought to play a role in depression and obsessive compulsive disorder. However, their functional transmitter repertoire is incompletely known. To investigate this repertoire, intracellular recordings were obtained from 132 cytochemically identified rat mesopontine serotonergic neurons that ha...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(94)90283-6
更新日期:1994-02-01 00:00:00
abstract::We have examined GABAA receptor activation by making rapid applications of GABA to outside-out patches excised from cultured postnatal rat cerebellar neurons. The rate of development of current increases with increasing GABA concentration from a low to a high concentration asymptote. The low concentration asymptote is...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(94)90152-x
更新日期:1994-01-01 00:00:00
abstract::Leukemia inhibitory factor (LIF) is a cytokine that affects the survival and differentiation of certain neuronal populations in vitro. To identify LIF-responsive neurons in the adult rat, we have demonstrated retrograde axonal transport of 125I-LIF to sensory and motor neurons. The accumulation of 125I-LIF by both cel...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(94)90163-5
更新日期:1994-01-01 00:00:00
abstract::We have examined the long-term effects of notochord ablation at chick stages 9-10 on formation of the floor plate and motor neurons. Although missing or reduced 2 days postablation, the floor plate and motor neurons were morphologically normal by 4 postoperative days. When isolated whole or ventral, but not lateral, n...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90227-i
更新日期:1993-12-01 00:00:00
abstract::The product of the Wnt-1 proto-oncogene is a secreted glycoprotein that is normally produced in regions of the embryonic neural tube. We show here that expression of mouse Wnt-1 cDNA in the rat PC12 pheochromocytoma cell line causes a dramatic conversion from a round to a flat cell morphology. In addition, PC12 cells ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90116-9
更新日期:1993-11-01 00:00:00
abstract::N-type calcium channels play a dominant role in controlling synaptic transmission in many peripheral neurons. Transmitter release from mammalian central nerve terminals, however, is relatively resistant to the N channel antagonist omega-conotoxin GVIA. We studied the sensitivity of glutamatergic synaptic transmission ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90119-c
更新日期:1993-11-01 00:00:00
abstract::Mutations in the retinal degeneration, retinal degeneration slow(/peripherin) and rhodopsin genes cause photoreceptor degeneration in humans and mice. Although the phenotypes arising from these mutations are different, suggesting different mechanisms of pathogenesis, we present evidence that apoptosis may be the final...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90072-y
更新日期:1993-10-01 00:00:00
abstract::Acetylcholinesterase (AChE) is concentrated at the vertebrate neuromuscular synapse. To determine whether increased transcript levels could underlie this selective accumulation, we employed a quantitative reverse transcription polymerase chain reaction-based assay to determine mRNA copy number in samples as small as s...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90151-g
更新日期:1993-09-01 00:00:00
abstract::The bendless (ben) mutation of Drosophila alters synaptic connectivity between a subset of CNS neurons. Here, we show that ben also causes morphological abnormalities within the visual system, suggesting that ben functions in a number of different developmental processes. We show that the ben gene encodes a protein wh...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90182-q
更新日期:1993-08-01 00:00:00
abstract::Involvement of the cAMP cascade in Drosophila learning and memory is suggested by the aberrant behavioral phenotypes of the mutants dunce (cAMP phosphodiesterase) and rutabaga (adenylyl cyclase). Line DCO581, isolated via an enhancer detector screen for genes preferentially expressed in the mushroom bodies, contains a...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90178-t
更新日期:1993-08-01 00:00:00
abstract::M-current is widespread in the nervous system. It stabilizes cell excitability, and its suppression by muscarinic receptor activation underlies slow synaptic transmission in sympathetic neurons. Suppression of M-current was one of the first examples of neuromodulation of a potassium current, but the mechanism is not u...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90272-s
更新日期:1993-07-01 00:00:00
abstract::We have investigated ubiquitinated paired helical filaments, which produce a proteinaceous smear in SDS-polyacrylamide gel electrophoresis and immunoblotting. The smear consisted largely of the carboxy-terminal portion of tau and ubiquitin. The ubiquitin-targeted protein was identified as tau in paired helical filamen...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90063-w
更新日期:1993-06-01 00:00:00
abstract::Argiotoxin, a component of the spider venom from Argiope lobata, blocks AMPA receptor channels expressed in homomeric and heteromeric configuration in Xenopus oocytes. Argiotoxin acts as an open channel blocker in a voltage-dependent manner and discriminates between the functionally diverse AMPA receptors. Importantly...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90061-u
更新日期:1993-06-01 00:00:00
abstract::Abnormally phosphorylated tau proteins (A68) are the building blocks of Alzheimer's disease (AD) paired helical filaments. The biological consequences of the conversion of normal adult tau to A68 remain unknown. Here we demonstrate that native A68 does not bind to microtubules (MTs), yet dephosphorylated A68 regains t...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90057-x
更新日期:1993-06-01 00:00:00
abstract::The modulation of high voltage-activated (HVA) Ca2+ currents by L-glutamate and its agonists was investigated in cultured rat hypothalamic neurons. L-Glutamate and agonists selective for NMDA or non-NMDA receptors reversibly inhibited HVA Ca2+ currents. The putative presynaptic glutamate receptor agonist L-2-amino-4-p...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90203-4
更新日期:1993-05-01 00:00:00
abstract::Insulin-like growth factor I (IGF-I) is a potent regulator of oligodendrocyte development and myelination in vitro, but its effect on myelination in vivo has never been tested directly. Therefore, we examined brain growth and myelination in a transgenic mouse line that overexpresses IGF-I. By postnatal day 55, when br...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90173-o
更新日期:1993-04-01 00:00:00
abstract::We have identified the species of K+ channel that underlies the neuronal M-current in rat sympathetic ganglion cells. The channels were kinetically and pharmacologically defined using outside-out and cell-attached patches. They exhibited multiple conductance levels, predominantly 3-9 pS. Their slow gating in response ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90166-o
更新日期:1993-04-01 00:00:00
abstract::A circadian clock regulates a number of diverse physiological functions in the vertebrate eye. In this study, we show that mRNA for the red-sensitive cone pigment, iodopsin, fluctuates with a circadian rhythm in chicken retina. Transcript levels increase in the late afternoon just prior to the time of cone disc sheddi...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90161-j
更新日期:1993-04-01 00:00:00
abstract::Quaternary ammonium (QA) compounds inhibit K+ conductance by entering and occluding the open pore of voltage-activated K+ channels. We characterized the effects of a series of alkyl-triethylammonium blockers on the Shaker K+ channel and tested them on a series of site-directed mutants of the channel protein in order t...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90340-w
更新日期:1993-03-01 00:00:00
abstract::Since the inflammatory process develops after transplantation to the brain, we sought to determine the presence of cytokines following a surgical trauma to the brain of an adult mouse. We report the early and marked presence of tumor necrosis factor-alpha and interleukin-1 alpha in neuronal somata of the striatum foll...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90312-f
更新日期:1993-02-01 00:00:00
abstract::Two mutations in the sodium channel alpha subunit that have been implicated as the cause of periodic paralysis were studied by functional expression in a mammalian cell line. Both mutations disrupted inactivation without affecting the time course of the onset of the sodium current or the single-channel conductance. Th...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90321-h
更新日期:1993-02-01 00:00:00
abstract::We have used the caged calcium compound DM-nitrophen to investigate the kinetics of calcium-dependent secretion in bovine chromaffin cells. Perfusion with partially calcium-loaded nitrophen often caused a loading transient--slow secretion for up to 1 min due to displacement of Ca2+ by cytoplasmic Mg2+. Flash photolysi...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90238-m
更新日期:1993-01-01 00:00:00
abstract::We recently proposed that ciliary neurotrophic factor (CNTF) shares two receptor components with a generally acting cytokine, leukemia inhibitory factor (LIF), but that CNTF also requires a third receptor component (CNTFR alpha) that is mostly restricted to the nervous system in its expression. Here we demonstrate tha...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90245-m
更新日期:1993-01-01 00:00:00
abstract::The distribution of the synaptic vesicle-associated phosphoprotein synapsin I after electrical stimulation of the frog neuromuscular junction was investigated by immunogold labeling and compared with the distribution of the integral synaptic vesicle protein synaptophysin. In resting terminals both proteins were locali...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90072-l
更新日期:1992-12-01 00:00:00
abstract::The N-myc and the L-myc proto-oncogenes are expressed during embryonal development mainly in the developing brain. Studies of their expression in single neuroepithelial cells revealed that neural precursors not yet committed to the glial or the neuronal lineage expressed both genes, but after lineage commitment they e...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90079-s
更新日期:1992-12-01 00:00:00
abstract::Two types of ryanodine receptors, channels for Ca2+ release from intracellular stores, are known. We detected the skeletal muscle type only in cerebellum by immunoblot analysis of microsomes and partially purified proteins. The cardiac muscle type was found in all parts of the mouse brain. Immunohistochemical study sh...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90071-k
更新日期:1992-12-01 00:00:00
abstract::The subunit stoichiometry of the mammalian K+ channel KV1.1 (RCK1) was examined by linking together the coding sequences of 2-5 K+ channel subunits in a single open reading frame and tagging the expression of individual subunits with a mutation (Y379K or Y379R) that altered the sensitivity of the channel to block by e...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90239-a
更新日期:1992-11-01 00:00:00
abstract::We show here that antisense MAP2 oligonucleotides inhibit neurite outgrowth in cultured cerebellar macroneurons. Unlike control neurons, which first extend a lamellipodial veil followed by a consolidation phase during which the cells extend minor neurites, MAP2-suppressed cells persist with lamellipodia and later beco...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90025-9
更新日期:1992-10-01 00:00:00
abstract::Two critical amino acids in the visual pigment rhodopsin are Lys-296, the site of attachment of retinal to the protein through a protonated Schiff base linkage, and Glu-113, the Schiff base counterion. Mutation of Lys-296 or Glu-113 results in constitutive activation of opsin, as assayed by its ability to activate tra...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90034-b
更新日期:1992-10-01 00:00:00
abstract::To investigate the role of intracellular Ca2+ in the survival of developing neurons before they become neurotrophic factor dependent, we have studied chick embryo nodose neurons, which have a particularly protracted period of neuorophic factor independence. Pharmacological reduction of intracellular free Ca2+ or deple...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90193-h
更新日期:1992-09-01 00:00:00
abstract::Calcium-activated potassium channels were expressed in Xenopus oocytes by injection of RNA transcribed in vitro from complementary DNAs derived from the slo locus of Drosophila melanogaster. Many cDNAs were found that encode closely related proteins of about 1200 aa. The predicted sequences of these proteins differ by...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90160-f
更新日期:1992-08-01 00:00:00
abstract::Charybdotoxin (CTX) blocks high conductance Ca(2+)-activated K+ channels by binding to a receptor site in the externally facing "mouth." Toxin bound to the channel can be destabilized from its site by K+ entering the channel from the opposite, internal, solution. By analyzing point mutants of CTX expressed in E. coli,...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90169-e
更新日期:1992-08-01 00:00:00